Table 1. X-ray diffraction data and refinement statistics.
Values in parentheses are for the highest resolution shell.
| Edited 10 × 10 | Unedited 10 × 10 | Edited 23 × 19 | |
|---|---|---|---|
| Data collection | |||
| Space group | H3 | H3 | P41212 or P43212 |
| Unit-cell parameters (Å) | a = 39.5, c = 246.4 | a = 49.0, c = 235.8 | a = 62.5, c = 63.6 |
| Resolution | 29.92–1.35 (1.40–1.35) | 23.38–2.85 (2.95–2.85) | 36.29–2.80 (2.90–2.80) |
| Total reflections | 122008 | 16052 | 16572 |
| Unique reflections | 30558 | 4798 | 3307 |
| Redundancy | 3.99 (1.78) | 3.35 (3.67) | 5.01 (4.82) |
| Completeness (%) | 96.8 (77.4) | 97.2 (99.8) | 97.0 (92.7) |
| 〈I/σ(I)〉 | 13.1 (2.2) | 10.3 (1.9) | 11.4 (2.2) |
| Rsym† (%) | 6.3 (35.4) | 6.1 (37.9) | 9.9 (33.4) |
| Twinning fraction | 0.38 | 0.41 | None |
| Refinement statistics | |||
| Resolution | 30.0–1.5 | ||
| No. of atoms (RNA/water) | 1257/26 | ||
| Rwork‡/Rfree§ (%) | 31.9/31.8 | ||
| R.m.s.d. bonds (Å) | 0.0038 | ||
| R.m.s.d. angles (°) | 0.94 |
R
sym =
× 100.
R
work =
× 100. R
work was calculated using 93% of the data.
R free is defined as the R work calculated using 7% of the data selected randomly and excluded from the refinement.