Table 2. Revised Fast Screen crystallization hit count by position.
| Hits | ||||||||
|---|---|---|---|---|---|---|---|---|
| Alanine | Glutamine | |||||||
| Position | Mutated | Secondary structure† | Exposure‡ | 277K | RT | 277K | RT | Total |
| 17 | A, Q | Helix | I | 0 | 2 | 9 | 2 | 13 |
| 76 | Coil | E | ||||||
| 78 | A, Q | Helix | I | 8 | 4 | 9 | 3 | 24 |
| 82 | Helix | I | ||||||
| 192 | A, Q | Sheet | I | 4 | 2 | 3 | 4 | 13 |
| 196 | A, Q | Coil | E | 6 | 3 | 14 | 5 | 28 |
| 203 | A, Q | Coil | E | 0 | 1 | 5 | 1 | 7 |
| 206 | A, Q | Sheet | E | 4 | 3 | 9 | 3 | 19 |
| 232 | A, Q | Sheet | I | 7 | 1 | 8 | 4 | 20 |
| 250 | A, Q | Helix | E | 7 | 1 | 8 | 5 | 21 |
| 283 | Helix | B | ||||||
| 301 | A, Q | Sheet | E | 4 | 2 | 9 | 6 | 21 |
| 304 | Disordered | NA | ||||||
| 312 | Sheet | E | ||||||
| 376 | Helix | E | ||||||
| 379 | Coil | I | ||||||
| 390 | Sheet | B | ||||||
| 392 | Coil | B | ||||||
| 404 | A, Q | Helix | E | 7 | 4 | 8 | 5 | 24 |
| 421 | Sheet | B | ||||||
| 473 | Helix | I | ||||||
| 477 | Helix | I | ||||||
| 483 | Q | Helix | E | 5 | 5 | 10 | ||
| 501 | Q | Coil | E | 2 | 1 | 3 | ||
| 506 | Sheet | B | ||||||
| 522 | Helix | B | ||||||
| 619 | Sheet | E | ||||||
| 653 | A, Q | Helix | E | 11 | 3 | 5 | 5 | 24 |
| 665 | A, Q | Helix | E | 8 | 3 | 11 | 4 | 26 |
| 691 | Helix | I | ||||||
| 702 | A, Q | Coil | E | 7 | 3 | 4 | 3 | 17 |
| 720 | Helix | I | ||||||
| Total§ | 73 | 32 | 109 | 56 | 270 | |||
†
Secondary-structural classification was performed using the program DSSP (Kabsch Sander, 1983 ▶). Both 310- and -helices are denoted as Helix; Coil corresponds to all secondary-structural elements except helices and sheets. Residue 304 was disordered in all structures.
‡
Disordered lysines were automatically modeled by the Swiss-Pdb Viewer (Guex Peitsch, 1997 ▶) and lysine side-chain solvent accessibility was calculated using the CCP4 program AREAIMOL, which was subsequently classified as B for buried, I for intermediate and E for exposed after Rost Sander (1994 ▶).
§
Wild-type E. coli malate synthase G produced seven crystals at 277K and five at room temperature and is not included in the table.