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. Author manuscript; available in PMC: 2007 Sep 17.
Published in final edited form as: Arch Biochem Biophys. 2007 Jan 23;461(2):200–210. doi: 10.1016/j.abb.2006.12.030

Figure 4.

Figure 4

NR box and AF1 squelching of E2-induced transactivation in cells transfected with pSp13. NR box inhibition of ERα/Sp1-mediated transactivation (A) and coactivation of ERα/Sp1 by DRIP150 (B) in ZR-75 cells. Cells were transfected with pSp13 and ERα alone or in combination with DRIP150, treated with DMSO or 10 nM E2, and luciferase activity was determined as described in the Materials and Methods. NR box squelching of DRIP150-mediated coactivation of ERα/Sp1 was determined by cotransfection of different amounts of the NR box expression plasmid (0 - 100 ng). Significant (p < 0.05) induction by E2 (*) and inhibition by NR box peptide expression are indicated (***). AF1 peptide inhibition of ERα/Sp1 (C, D). Cells were transfected and treated as described in A/B. Squelching of DRIP150 coactivation of ERα/Sp1 was determined by cotransfection with different amounts (0 - 100 ng) of an expression plasmid expressing the AF1 domain of ERα [49]. Significant (p < 0.05) inhibition of ERα/Sp1-mediated transactivation by the AF1 expression plasmid is indicated (**). Significant (p < 0.05) reversal of AF1-dependent inhibition by DRIP150 (D) is also indicated (***).