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. Author manuscript; available in PMC: 2007 Sep 17.
Published in final edited form as: Arch Biochem Biophys. 2007 Jan 23;461(2):200–210. doi: 10.1016/j.abb.2006.12.030

Figure 5.

Figure 5

Interactions of DRIP150, ERα and Sp1. (A, B) Coimmunoprecipitation of DRIP150 and Sp1 alone (A) or plus ERα (B). 35S-Labeled protein or empty vector (35SpcDNA3.0) were coincubated with various antibodies and immunoprecipitates were analyzed by SDS-PAGE as described in the Materials and methods. (C) Gel mobility shift assay. 32P-Labeled Sp1 oligonucleotide was incubated with one or more proteins (ERα, Sp1 and DRIP150), Sp1 or non-specific IgG antibodies and oligonucletides and analyzed by gel mobility shift assays as described in the Materials and Methods. The specifically-bound Sp1-DNA complex and the antibody supershifted complex (SS) are indicated.