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. Author manuscript; available in PMC: 2007 Sep 17.
Published in final edited form as: Arch Biochem Biophys. 2007 Jan 23;461(2):200–210. doi: 10.1016/j.abb.2006.12.030

Figure 7.

Figure 7

Squelching of DRIP150 coactivation of ERα/Sp1. (A) Wild-type and mutant GAL4-pM23 construct used in squelching experiments. Overexpression of pM23 (B), pM23 A792P (C), pM23R801P(D) and pM23A792P/R801P (E) on coactivation of ERα/Sp1 by DRIP150. ZR-75 cells were transfected with pSp13/ERα, different amounts of pM23-derived constructs, treated with DMSO or 10 nM E2, and luciferase activity was determined as described in the Materials and Methods. Significant (p < 0.05)induction by E2 (*), coactivation by DRIP150 (**), and squelching of this response by wild-type and mutant pM23 peptides (***) are indicated.