Table 3.
In vivo inhibition of P. berghei oocyst formation by gomesin
| Exp. | Gomesin concentration | Oocysts per Midguta | Oocyst-positive mosquitoesb | % Oocyst inhibition |
|---|---|---|---|---|
| 1 | 25 μM
control |
72.1 (0–225)
131.4 (0–352) |
93.3 (14/15)
91.7 (11/12) |
45 |
| 2 | 50 μM
Control |
14.5 (0–162) *
30.8 (0–122) |
65.6 (21/32)
83.3 (25/30) |
53 |
| 3 | 50 μM
Control |
74.4 (0–244)*
375.8 (190–592) |
94.1 (16/17)
100 (5/5) |
80 |
| 4 | 100 μM
Control |
2.4 (0–14)**
17.6 (0–72) |
40 (14/35)
85.7 (30/35) |
86 |
| 5 | 200 μM
Control |
85.3 (3–188) *
274 (95–510) |
100 (11/11)
100 (5/5) |
69 |
| 6 | 200 μM
Control |
19.2 (0–92) *
65.7 (0–284) |
97 (33/34)
81.3 (26/32) |
70 |
For each experiment, control An. stephensi mosquitoes were fed on anaesthetized P. berghei-infected mouse. A second group of experimental mosquitoes was fed on the same mouse about 10 min after injection of gomesin into its tail vein. Mosquitoes were kept at 21°C and the number of oocysts per midgut was determined on day 15. Data from 6 independent experiments.
Mean oocyst number per mosquito midgut. The range of observed values is indicated in parentheses.
Percentage of mosquitoes that had oocysts in their midgut. These values were derived from the number of oocyst-positive mosquitoes over the total number of mosquitoes examined (shown in parentheses).
p<0.05;
p<0.0001 in comparison to the control by the Mann-Whitney test.