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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Anal Biochem. 2006 Dec 28;363(1):160–162. doi: 10.1016/j.ab.2006.12.013

Figure 1.

Figure 1

Dexamethasone-trimethoprim (Dex-TMP) yeast three-hybrid system. A heterodimeric ligand (Dex-TMP) bridges a DNA-binding domain-dihydrofolate reductase fusion protein (DBD-DHFR) and a transcriptional activator domain-glucocorticoid receptor fusion protein (AD-GR), effectively reconstituting a transcriptional activator (DBD-AD) and activating transcription of a downstream reporter gene.