Figure 4.
Inhibition of LPS-activated p-IκBα protein expression and NF-κB activation by MCE. The level of the p-IκBα protein (A) was monitored for 15 min after treating the cells with LPS (1 μg ml−1) with or without the MCE pretreatment (i.e. 1 h before LPS treatment). NF-κB activation (B) from the nuclear extracts was analyzed by gel shift assay. Each lane contained 5 μg of the nuclear extracts. The results were confirmed by repeated analyses.