Table 1.
Electrophysiological parameters and responses to basolateral stimuli in untransfected Colony 1 cells and sst2 receptor clones
| Basal R | Basal ISC | VIP 30 nM ΔISC |
SRIF-14 |
UK14,304 | ||
|---|---|---|---|---|---|---|
| Cells | (Ω cm2) | (μA cm−2) | (μA cm−2) | pEC50 | 100 nM ΔISC (μA cm−2) | 1 μM ΔISC (μA cm−2) |
| Colony 1 | 39.3±0.6 (531) | 11.0±0.4 (531) | +29.5±1.2 (200) | — | 0.0±0.0 (14) | −10.4±0.9 (4) |
| hS2a | 131.2±4.1 (125) | 9.9±4.9 (125) | +22.1±1.0 (103) | 7.64±0.07 | −9.6±1.1 (8) | −5.8±0.5 (6) |
| rS2a | 100.1±2.5 (237) | 50.6±1.7 (237) | +73.2±1.8 (189) | 7.67±0.07 | −22.4±2.2 (6) | −25.3±3.4 (6) |
| rS2b | 63.4±2.8 (126) | 25.2±1.1 (126) | +83.0±3.3 (105) | 7.86±0.07 | −24.3±4.4 (6) | −19.5±3.6 (6) |
Abbreviations: SRIF-14, somatotrophin release inhibiting factor; UK14,304, 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine; VIP, vasoactive intestinal polypeptide.
pEC50 values for SRIF-14 were calculated from pooled single addition concentration–response relationships for the inhibition of 30 nM VIP-stimulated ISC (n=3–8).
Values in parentheses indicate the number of observations for basal resistances (R) and ISC, and for the change in ISC (ΔISC) after each agonist addition at the optimal concentrations shown.