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. 2007 Jul 2;152(1):122–131. doi: 10.1038/sj.bjp.0707368

Figure 7.

Figure 7

Effects of ML-9 and ML-7 on single TRPC6 channels. Inside-out (I/O) patch recording with CCh (100 μM) in the pipette. To suppress K+ and Cl− channels, 5 mM tetraethylammonium and 100 μM DIDS were also added in the pipette. (a) Actual traces at three different potentials (−50, 0 and 50 mV). Boxes on the right indicate all-points-in-open-state amplitude histograms with the results of Gaussian fit (smooth curves). (b) I–V relationship for single TRPC6 channel. Linear regression of data points between −50 and 50 mV gives a unitary conductance of 33.4 pS (n=5–8), which is in good accordance with the value obtained previously (Shi et al., 2004). (c) Inhibition of single TRPC6 channel activity by 10 μM ML-9, which was applied rapidly into the bath at the bar. Two large vertical deflections indicate the electrical artefacts of a solenoid valve. (d) Summary of the inhibitory effects of 10 μM ML-9 and ML-7 on single TRPC6 channels. The extent of single-channel current inhibition by ML compounds was calculated as the ratio of mean currents (averaged for 2 s) 5 s after, to just before, application of the drugs. n=8–11. CCh, carbachol; DIDS, [4,4-diisothiocyanostilbene-2,2-disulphonic acid, 2Na]; ML-7, [1-(5-iodonaphthalene-1-sulphonyl)homopiperazine, HCl]; ML-9, [1-(5-chloronaphthalene-1-sulphonyl)homopiperazine, HCl].