Table 1.
Antiproliferative activity of the NO-donors on epimastigote forms at different concentrations and time of incubation
|
Antiproliferative activity (% GI) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| T=1 h |
T=4 h |
T=24 h |
||||||||
| NO donors |
Concentrations (mM) |
|||||||||
| 0.1 | 0.5 | 1 | 0.1 | 0.5 | 1 | 0.1 | 0.5 | 1 | IC50epi (μM) | |
| t-[Ru(NO)(NH3)4pz]3+ | 30±4 | 66±5 | 87±3 | 73±4 | 85±3 | 90±9 | 89±4 | 92±5 | 100 | 56 |
| t-[Ru(NO)(NH3)4isn]3+ | 15±4 | 19±3 | 70±6 | 27±5 | 55±4 | 72±2 | 75±2 | 86±4 | 91±7 | 67 |
| t-[Ru(NO)(NH3)4L-Hist]3+ | 31±3 | 49±3 | 57±7 | 36±2 | 60±8 | 62±4 | 68±5 | 71±4 | 77±5 | 78 |
| t-[Ru(NO)(NH3)4imN]3+ | 16±6 | 37±4 | 54±3 | 35±5 | 39±2 | 65±4 | 58±4 | 65±4 | 78±4 | 86 |
| t-[Ru(NO)(NH3)4py]3+ | 12±9 | 55±5 | 62±6 | 26±4 | 47±3 | 65±4 | 56±6 | 78±4 | 85±4 | 90 |
| t-[Ru(NO)(NH3)4nic]3+ | 25±5 | 63±3 | 72±4 | 39±2 | 61±6 | 89±8 | 47±5 | 81±4 | 89±6 | 136 |
| SNP | 19±5 | 35±2 | 51±4 | 22±5 | 56±4 | 65±3 | 41±4 | 66±6 | 74±4 | 244 |
| [Ru(NO)(Hedta)] | 28±4 | 50±4 | 56±8 | 20±3 | 50±4 | 56±5 | 33±2 | 72±7 | 89±4 | 275 |
| t-[Ru(NO)(NH3)4P(OEt)3]3+ | 57±4 | 68±9 | 51±3 | 12±3 | 35±4 | 58±4 | 25±4 | 69±6 | 74±5 | 328 |
Abbreviation: NO, nitric oxide.
Results are mean±s.e.m., n=4–6, P<0.05. Antiproliferative activity expressed as the percentage of growth inhibition at the concentrations shown. IC50epi corresponds to the concentration with 50% antiproliferative activity, after 24 h of incubation.