Table 3.

Effects of EGIS-8332 and GYKI 53405 on pyramidal cell death in the CA1 area of the hippocampus, spontaneous alternation response and locomotor activity measured in a Y-maze following a 3-min BCO in Mongolian gerbils

Groups	Ischaemic damage (score)	Spontaneous alternation (% of all entries)	Locomotor activity (number of arm entries)
Sham operation		72.3±2.7	35.1±1.8
BCO	4.87±0.14	51.1±2.5+++	49.0±3.2+
BCO+EGIS-8332, 10 mg kg−1 i.p.	3.00±0.59**	54.9±3.5++	39.4±3.7
Sham operation		58.5±2.0	42.6±3.0
BCO	4.71±0.18	51.4±5.9	60.1±5.4+
BCO+EGIS-8332, 20 mg kg−1 i.p.	1.57±0.65**	54.9±3.6	41.6±6.4*
Sham operation		57.6±3.3	38.5±3.0
BCO	4.90±0.10	54.6±2.9	60.7±2.8++
BCO+GYKI 53405, 10 mg kg−1 i.p.	3.20±0.59**	56.5±4.2	41.7±4.4**
Sham operation		62.3±3.5	36.5±3.2
BCO	4.70±0.21	46.8±3.0+	57.7±4.3++
BCO+GYKI 53405, 20 mg kg−1 i.p.	2.22±0.76**	49.2±2.8+	43.8±4.1*

Abbreviations: ANOVA, analysis of variance; BCO: bilateral carotid artery-occluded, vehicle-treated animals; i.p., intraperitoneal; Sham: sham-operated, vehicle-treated animals.

The compounds or vehicle were administered 45 min after BCO (n=8–15/group). Behavioural variables were measured on day 4 after surgery.

Data shown in the table are means±s.e.m.; EGIS-8332 and GYKI 53405 were administered after BCO at doses indicated.

⁺P<0.05,

⁺⁺P<0.01,

⁺⁺⁺P<0.001 compared with the sham-operated group.

^*P<0.05,

^**P<0.01 compared with the BCO group (Kruskal–Wallis ANOVA followed by Mann–Whitney U-test).