Figure 2.
Histochemical staining for β-glucuronidase activity in the liver of an untreated MPS VII mouse (A and B) shows no enzyme activity. Sixteen days after receiving 1.79 × 109 pfu of recombinant adenovirus, the liver from an MPS VII mouse (C and D) shows numerous β-glucuronidase-containing cells. Their distribution and location suggest that many are sinus lining cells. Scattered large polygonal cells morphologically consistent with hepatocytes also contained β-glucuronidase. Thirty-five days after receiving 1.79 × 109 pfu of recombinant adenovirus (E and F), β-glucuronidase-containing cells are still seen, although they are less numerous than at 16 days after injection. (A, C, and E, ×20; B, D, and F, ×210.)