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. 2007 Sep 20;117(11):3475–3488. doi: 10.1172/JCI31743

Figure 5. Lack of endogenous Mc4r affects WAT metabolism.

Figure 5

(A) Relative abundance of LPL mRNA in WAT of Mc4r-KO and WT mice. FAS, SCD-1, and ACC were also analyzed but did not show statistical difference (data not shown). Data are presented as values normalized to the housekeeping gene HPRT. Values are mean ± SEM of 6–8 animals per group. *P < 0.05. (B and C) Basal and insulin-stimulated PKB activity as assessed by Western blot analysis of Ser473 phosphorylation of PKB (B) and measurement of PKB kinase activity (C) was increased in WAT of Mc4r-KO mice compared with WT littermates. Phosphorylation of FoxO1 on Ser256, an important transcription factor target of PKB, was also increases in the basal and insulin-stimulated state in Mc4r-KO mice. For the experiments represented in B and C, 6-week-old pre-obese Mc4r-KO mice and WT B6 controls were fasted overnight and then administered saline or 1 U/kg insulin, and PKB and FoxO1 phosphorylation and/or activity were measured as described previously (42).