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. 2006 Sep 11;149(4):355–364. doi: 10.1038/sj.bjp.0706883

Figure 6.

Figure 6

Western blot analysis of the expressions of MYPT1 and p-MYPT1 in BAECs and BASMCs. Total cellular proteins were prepared as described in the legend to Figure 5. Basal expression levels of MYPT1 and β-actin before ATP application are shown in (a) band images show data from a representative experiment and the bars show the densitometric analysis of MYPT1 expression relative to β-actin from six experiments (*P<0.05, **P<0.01). Expression of p-MYPT1 before, during and after ATP (10 μM) application are shown in (b). Band images show a representative result and the graph shows the time-dependent change in p-MYPT1/total MYPT values, relative to the initial (time=0) value (n=5).