Figure 2.

Metabolism of cADPR and N1-cIDPR by CD38. Vehicle (no substrate), cADPR (50 μM) or N1-cIDPR (50 μM) were incubated at RT either with 1 × 10⁷ ml⁻¹ Jurkat T-lymphocytes for the times indicated (a–i, j), with recombinant soluble mouse CD38 (0.75 μg ml⁻¹) (k) or with increasing numbers of Jurkat T-lymphocytes for 6 h (l). Aliquots were taken at the time points indicated and were analysed by RP-HPLC. The detection was performed at 270 nm for cADPR and at 250 nm for N1-cIDPR. Data are presented as mean±s.d. (n=3). Note that at some time points s.d. values are smaller than symbols and thus cannot be seen properly.