Figure 5.

Comparative effects of (a) pyrrophenone, (b) MAFP and (c) AACOCF₃ on A23187- and AA-induced 5-LO product biosynthesis in human PMN. PMN suspensions (37°C, 5 × 10⁶ cells ml⁻¹) were treated with ADA (0.3 U ml⁻¹) and increasing concentrations of cPLA₂α inhibitors 10 min before stimulation with either 100 nM A23187 or 2.5 μM AA for 5 min as described in Methods. Incubations were stopped by the addition of 0.5 volume of cold (4°C) stop solution (MeOH/MeCN, 50/50, v/v) containing 12.5 ng of both 19-OH-PGB₂ and PGB₂ as internal standards. 5-LO products were analyzed as described in Methods. Data represent the mean (±s.e.m.) of three separate experiments, each performed in duplicate.