Figure 6.

Effect of pyrrophenone on PLD activity in activated PMN. PMN suspensions (37°C, 10⁷ cells ml⁻¹) were labeled with 1-O-[³H]alkyl-2-lyso-phosphatidylcholine. Cells were then stimulated for 10 min with either 1 nM fMLP or 100 nM thapsigargin in the presence of 1% EtOH (final concentration). Incubations were stopped by the addition of 3.6 volumes of a cold (4°C) stop solution (CH₃Cl/MeOH/HCl, 50/100/1, v/v/v) containing unlabeled 1-O-alkyl-2-lyso-phosphatidylethanol as internal standard. 1-O-[³H]alkyl-2-lyso-phosphatidylcholine and phosphatidylethanol were separated by TLC and analyzed for radioactivity content as described in Methods. Data represent the mean (±s.e.m.) of three separate experiments.