Fig. 13.
Phosphorylation of ERK1/2, JNK1/2, p38, and protein kinase B/Akt during TBHP exposure and recovery of RPTC following TBHP exposure. Left: sublethal injury (mitochondrial dysfunction without cell death) induced by 0.35 mM TBHP. Middle: lethal injury (mitochondrial dysfunction followed by 70% cell death) induced by 0.50 mM TBHP. Right: effect of MEK1/2 inhibitor, 10 μM U0126, on ERK1/2, JNK1/2, p38, and protein kinase B/Akt phosphorylation following exposure to 0.5 mM TBHP. TBHP exposure had no effect on total protein levels of protein kinases shown in this figure. The blots are representative of 3 independent experiments.