Table 1. Oligonucleotides used in this study.
Method | Symbol | Oligonucleotide |
Morpholino | ||
Control | con MO | 5′ CCTCTTACCTCAGTTACAATTTATA |
int6 Translation block | int-6 MO | 5′ GGTCAGATCGTACTCCGCCATGATG |
int6 5-base pair mismatch | int-6 5MM | 5′ GGTgAGATCcTAgTCCGCgATcATG |
siRNA | ||
INT6 sense siRNA (si) | INT6-siRNA | 5′ GAACCACAGUGGUUGCACAUU |
INT6 reverse siRNA (R) | R | 5′ UUACACGUUGGUGACACCAAG |
RT-PCR primers | ||
MEK1 forward | 5′ ATTATTGTTCCCCTAAGTGGATTG | |
MEK1 reverse | 5′ TTACAACAGCATTGGTACTTGGAT | |
INT6 forward | 5′ ATGGCGGAGTACGACTTGACT | |
INT6 reverse | 5′ TCAGTAGAAGCCAGAATCTTGAGT | |
Actin forward | 5′ CGTGATGGTGGGCATGGGTCA | |
Actin reverse | 5′ CTTAATGTCACGCACGATTTCC |