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. 2007 Sep 26;2(9):e959. doi: 10.1371/journal.pone.0000959

Table 1. Oligonucleotides used in this study.

Method Symbol Oligonucleotide
Morpholino
Control con MO 5′ CCTCTTACCTCAGTTACAATTTATA
int6 Translation block int-6 MO 5′ GGTCAGATCGTACTCCGCCATGATG
int6 5-base pair mismatch int-6 5MM 5′ GGTgAGATCcTAgTCCGCgATcATG
siRNA
INT6 sense siRNA (si) INT6-siRNA 5′ GAACCACAGUGGUUGCACAUU
INT6 reverse siRNA (R) R 5′ UUACACGUUGGUGACACCAAG
RT-PCR primers
   MEK1 forward 5′ ATTATTGTTCCCCTAAGTGGATTG
   MEK1 reverse 5′ TTACAACAGCATTGGTACTTGGAT
   INT6 forward 5′ ATGGCGGAGTACGACTTGACT
   INT6 reverse 5′ TCAGTAGAAGCCAGAATCTTGAGT
   Actin forward 5′ CGTGATGGTGGGCATGGGTCA
   Actin reverse 5′ CTTAATGTCACGCACGATTTCC