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. Author manuscript; available in PMC: 2007 Sep 18.
Published in final edited form as: J Neurobiol. 2006 Mar;66(4):319–331. doi: 10.1002/neu.20232

Figure 3.

Figure 3

Organ of Corti explants treated with β-bungarotoxin and subjected to TUNEL assay. Organ of Corti of C57BL/6 mouse was treated with 0.5 μM β-bungarotoxin for 24 h (A). The treated sample was fixed and tested for TUNEL staining using FITC-labeled dUTP (green) and immunostained with parvalbumin 3 antibody and Cy5-conjugated secondary antibody (blue). (B) Organ of Corti of C57BL/6 mouse treated with control label solution (TUNEL CTRL) lacking terminal deoxynucleotidyl transferase, immunostained with parvalbumin 3 and Cy5-conjugated secondary antibody (blue). (C) A C57BL/6 mouse organ of Corti without β-bungarotoxin treatment was stained by the TUNEL procedure with FITC-labeled dUTP (green) and immunostained with parvalbumin 3 antibody (blue). The β-bungarotoxin treatment (A) caused neuronal cell death without death of the other cell types in the organ of Corti. Scale bars are 100 μm. [Color figure can be viewed in the online issue, which is available at http://www.interscience.wiley.com.]