FIGURE 6.

IL-2 inhibits Ag-specific T cell cycle progression while promoting the development of IL-4-producing T cells. Five million purified WT DO11.10 CD4⁺ T cells were labeled with CFSE and transferred to WT BALB/c mice. Two days later, recipient mice were administered 2 mg of anti-IL-2 (S4B6) or control Ab GL117 and immunized intracutaneously in the ear with N. brasiliensis (Nb) and OVA peptide (five animals per treatment group). At day 7 after immunization, the draining CLN cells were collected. a, Analysis of DO11.10 T cell cycling was performed as described in Fig. 1. b, The number of DO11.10 T cells in each CLN was calculated based on multiplying the percentage of KJ1-26⁺CD4⁺ cells measured by flow cytometry by the number of live cells in each CLN. c, Ex vivo cytoplasmic IL-4 staining was performed as described in Fig. 2. Data shown are with gated CD4⁺KJ1-26⁺ OVA-specific T cells. This experiment was repeated twice with similar results.