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. Author manuscript; available in PMC: 2008 May 29.
Published in final edited form as: Neurosci Lett. 2007 Apr 21;419(2):172–177. doi: 10.1016/j.neulet.2007.04.037

Figure 4.

Figure 4

SPARC increases galectin-3 secretion and cleavage. A and C. Immunoblot and densitometric analysis demonstrating no difference in galectin-3 levels in lysates of SPARC- (A2bi, A2b2) versus control- (B1b2, C2a2) transfected cells. Actin (A) is the loading control used for normalization in (D). B and D. Immunoblot and densitometric analysis showing the increase of full-length and both cleaved forms of galectin-3 in conditioned media from SPARC versus control-transfected cells. The relative levels of the 31-kDa (black), the 27-kDa (grey), and the 22-kDa (white) proteins are illustrated (D). The bars in total indicate relative levels of total secreted galectin-3 by each cell line. Representative results from n = 3 experiments.