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Construction of the α-tubulin gene (ATU) knockout plasmid. All plasmids are pBluescript-based constructs. pTUB100E3 contains a cloned HindIII fragment of the ATU gene that is marked by a silent HaeIII site; p4T2–1 contains the neo gene coding sequence; in pTUB-KO (the ATU gene knockout construct), the coding region of ATU is replaced by the coding region of neo. See text for description.
