Table 3.
Dependence of the abundance of the (bn−1 + H2O) ion on the position of an arginine, lysine or histidine residue.a
| Sequence | Position of underlined basic residueb | (bn−1 + H2O) abundance | Peptide source | |
|---|---|---|---|---|
| Peptides containing a single arginine residue (lysine and histidine absent): | ||||
| ATQEEFNR | 1 | ND | JGMV-IS | 121-128 |
| NLTDFNLAR | 1 | ND | JGMV-IS | 225-233 |
| GNVGESQENTER | 1 | W | JGMV-IS | 273-284 |
| FGLDGNVGESQENTER | 1 | W | JGMV-IS | 269-284 |
| MFGLDGNVGESQENTER | 1 | W | JGMV-IS | 268-284 |
| NLTDFNLARY | 2 | S | JGMV-IS | 225-234 |
| ATQEEFNRWY | 3 | S | JGMV-IS | 121-130 |
| FNRWYN | 4 | S | JGMV-IS | 126-131 |
| ATQEEFNRWYN | 4 | S | JGMV-IS | 121-131 |
| FNLARYAFD | 5 | S | JGMV-IS | 229-237 |
| SLARYAFDFY | 7 | S | JGMV-NI | 221-230 |
| NLTDFNLARYAFDFYE | 8 | S | JGMV-IS | 225-240 |
| DEQMRILMNGLMVWCIENGTSP | 18 | M | JGMV-NI | 132-153 |
| GRLNGAPALPNNGQYFIEAPQ | 20 | S | FoMV | 197-217 |
| Peptides containing a single non-C-terminal arginine in addition to just a C-terminal lysine residue: | ||||
| WYNRIK | 3 | S | JGMV-Aus | 130-135 |
| WYNRIK | 3 | S | JGMV-NI | 120-125 |
| FWYNRIK | 3 | S | JGMV-NI | 119-125 |
| @SGNEDAGRQK | 3 | S | JGMV-Aus | 1- 10 |
| HTQFQFWYNRIK | 3 | S | JGMV-NI | 114-125 |
| PIIPRGFDK | 5 | S | WSMV-IHC | 200-208 |
| RQVNEIFK | 8 | S | FoMV | 167-174 |
| MRLPMVSNK | 8 | S | JGMV-NI | 81- 89 |
| RGLTPAAFVQAAIIFTMESMDK | 22 | S | FoMV | 51- 72 |
| Peptides containing single arginine plus one or more non-C-terminal histidine residues only: | ||||
| ATHTQFQFWYNRIK | 3 | S | JGMV-Aus | 122-135 |
| NVHTYRGAK | 4 | S | JGMV-NI | 284-292 |
| HHFSDAAEAYIEYRNSK | 4 | S | JGMV-IS | 197-213 |
| DGNVGESSENTERHTAA | 5 | S | JGMV-NI | 263-279 |
| AAAIRGSTNHMF | 8 | S | JGMV-Aus | 259-270 |
| AREAHAQMK | 8 | S | JGMV-NI | 240-248 |
| AAAIRGSTNHMFGL | 10 | S | JGMV-NI | 249-262 |
| NASPTLRQIMHHFSDAAE | 12 | S | JGMV-IS | 187-204 |
| AVVVHLEVEHVRPTFDDFFTPVY | 12 | S | BMV | 166-188 |
| Peptides containing single arginine plus one or two non-C-terminal lysine residues only: | ||||
| FWYNRIKK | 4 | S | JGMV-NI | 119-126 |
| ATHTQFQFWYNRIKK | 4 | S | JGMV-NI | 112-126 |
| Peptides containing single arginine plus one or more non-C-terminal lysine or histidine residues | ||||
| NVHTYRGAKI | 5 | S | JGMV-NI | 284-293 |
| ATHTQFQFWYNRIKKEY | 6 | S | JGMV-NI | 112-128 |
| FNRWYNAIKKE | 9 | S | JGMV-IS | 126-136 |
| Peptides containing one or more non-C-terminal lysines plus a C-terminal arginine residue: | ||||
| NLNDKSLAR | 5 | M | JGMV-Aus | 226-234 |
| YTKPAYANR | 7 | M | FoMV | 109-117 |
| FYTKPAYANR | 7 | M | FoMV | 108-117 |
| TMMDGETQVTYPLKPVVENASPTLR | 12 | S | JGMV-IS | 169-193 |
| GETQVTYPLKPVVENASPTLR | 12 | S | JGMV-IS | 173-193 |
| EFPLKPIVENAKPTLR | 5,12 | M | JGMV-NI | 169-184 |
| SEFPLKPIVENAKPTLR | 5,12 | M | JGMV-NI | 168-184 |
| DQSEFPLKPIVENAKPTLR | 5,12 | S | JGMV-Aus | 176-194 |
| GNNQSEFPLKPIVENAKPTLR | 5,12 | M | JGMV-NI | 164-184 |
| IKKEYDVDDEQMR | 11,12 | M | JGMV-Aus | 134-146 |
| Peptides containing one or more non-C-terminal histidines plus a C-terminal arginine residue: | ||||
| HFSDAAEAYIEMR | 12 | S | JGMV-NI | 189-201 |
| QCMMHFSDAAEAYIEMR | 12 | W | JGMV-NI | 185-201 |
| GSTNHMFGLDGNVGESSENTER | 17 | W | JGMV-NI | 254-275 |
| QIMHHFSDAAEAYIEYR | 18,19 | W | JGMV-IS | 194-210 |
| Peptides containing non-C-terminal lysine and histidine plus a C-terminal arginine residue | ||||
| HLIQYKPDQR | 5,10 | M | JGMV-Aus | 106-115 |
| AILNLDHLIQYKPDQR | 5,10 | M | JGMV-Aus | 100-115 |
| Arginine-less peptides containing one or more lysine residues: | ||||
| YAFDFYEITSK | 1 | W | JGMV-IS | 234-244 |
| VSANDQSEFPLKPI | 3 | W | JGMV-Aus | 172-185 |
| WTMVSANDQSEFPLKPI | 3 | S | JGMV-Aus | 169-185 |
| SATPAENQPASADGKPAQTTATS | 9 | W | JGMV-KS | 11- 33 |
| DVDVGSTGTFVIPKLK | 1,3 | M | JGMV-NI | 60- 75 |
| DQSEFPLKPIVENAK | 1,8 | S | JGMV-Aus | 176-190 |
| AIKAIAGYSISK | 1,10 | W | BMV | 42- 53 |
| VVNNAGKDNEQQLEFK | 1,10 | M | WSMV-IHC | 149-164 |
| SATPAANQTASGDGKPAQTTATAENK | 1,12 | M | JGMV-Aus | 11- 36 |
| DKDVDVGSTGTFVIPK | 1,15 | M | JGMV-Aus | 68- 83 |
| SQTESQDKETGESVNKDK | 1,3,11 | S | JGMV-IS | 11- 28 |
| VVNNAGKDNEQQLEFKIEPMYK | 1,7,16 | S | WSMV-IHC | 149-170 |
| DKIKPEMINNMIK | 1,10,12 | M | WSMV-IHC | 72- 84 |
| ASTATKDKDVDVGSTGTFVIPK | 1,15,17 | S | JGMV-KS | 62- 83 |
| SGGTKASTATKDKDVDVGSTGTFVIPK | 1,15,17,23 | S | JGMV-KS | 57- 83 |
| Arginine-less peptides containing one or more histidine residues: | ||||
| GSTNHMFGL | 5 | W | JGMV-NI | 254-262 |
| GSTNHMFGLDGNVGE | 11 | W | JGMV-NI | 254-268 |
| NMHSLLGVQQSH | 1,10 | M | JGMV-IS | 293-304 |
| Arginine-less peptides containing lysine and histidine residues: | ||||
| DHLIQYKP | 2,7 | W | JGMV-NI | 95-102 |
| PHELSSEK | 1,7 | M | BMV | 74- 81 |
| PHELSSEKNK | 1,3,9 | M | BMV | 74- 83 |
ND = not detected
a
Basic residues are underlined. Sequences containing more than one arginine or no basic residues are not included.
b
Position of residue counting from the C-terminus