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. 1997 Feb 18;94(4):1361–1365. doi: 10.1073/pnas.94.4.1361

Figure 2.

Figure 2

PHA activation of CD25− PBMCs. CD25− quiescent PBMCs were purified by treatment with RFT5-dgA for 6 days. Cells were then thoroughly washed to remove RFT5-dgA and cultured in CM containing 5 μg/ml PHA. Intact cells were harvested 6 days after PHA stimulation and subjected to intact-cell PCR as described. HIV-1-infected unfractionated PBMCs were used as controls and β-actin served as the internal control. PHA-stimulated HIV-1-infected CD25− quiescent cells are shown in lanes 2, 4, 6, 8, 10, 12, 14, and 16. Unfractionated HIV-1 infected control cells are shown in lanes 1, 3, 5, 7, 9, 11, 13, and 15.