Figure.

(B) The role of adherent cells in the infection of CD25⁻ cells with HIV-1. Fresh PBMCs were prepared as described. PBMCs were cultured either in CM (lanes 1, 4, and 7) or in CM + RFT5-dgA (lanes 2, 5, and 8) for 6 days, and then exposed to HIV-1 for 2 hr and cultured in CM for 6 additional days, and then harvested and analyzed by intact-cell PCR. The 8E5/LAV cell line was used as positive control (lanes 3, 6, and 9). Lanes: 1–3, early transcripts (RU5); 4–6, full-length DNA; 7–9, β-actin as internal control.