Table 1.
Spleen cell number, CD4/CD8 ratio, and LPS response 1–3 months after bone marrow transplant with cytotoxically defective allogeneic T cells
| BM Transplant | Cells/spleen* | CD4/CD8 ratio† | LPS Response‡ |
|---|---|---|---|
| C3H.SW → C3H.SW | 22.9 × 106–100.0 × 106 | 5.1–5.6 | 169.4 |
| B6 → C3H.SW | 0.3 × 106–11.4 × 106 | 0.9–1.0 | 7.4 |
| B6-perforin 0/0 → C3H.SW | 1.6 × 106–11.9 × 106 | 1.0–1.1 | 5.3 |
| B6-gld → C3H.SW | 25.0 × 106–109.0 × 106 | 1.1–1.4 | 145.1 |
Total numbers of splenocytes recovered from recipient spleens at 33–77 days after transplantation of MHC-matched allogeneic bone marrow and 2.5 × 106 cytotoxically defective donor T cells. Recovery numbers are expressed as number of spleen cells per mouse.
CD4/CD8 ratios are calculated based on flow cytometric analysis of recipient spleen cells in at least three separate experiments for each group.
Values shown are the stimulation index values based on [3H]thymidine incorporation data from two separate representative experiments. Incorporation values were averaged and standard deviations for LPS-stimulated cultures ranged from 1.5% to 15.4%. Similar results were observed in 11/12 separate lymphocyte stimulation assays.