Figure 2.

PCR-amplified TCRBV2 transcripts from different sources were analyzed by SSCP. Lanes: 1, AB-TIL grown with IL-2 alone; 2, AB-TIL separately derived from a tissue fragment and grown with IL-2 and anti-CD3; 3, AB-TIL separately derived from a tissue fragment and grown with anti-CD3,8 bispecific mAbs and enriched for CD4⁺ cells; 4, freshly frozen tumor tissue from the shoulder metastatic lesion; 5, same as in lane 4 (duplicate conditions from a separate tumor tissue fragment); 6, AB-TIL separately derived from a tissue fragment and grown with anti-CD3,4 bispecific mAbs and highly enriched for CD8⁺ cells; 7, SH-TIL grown with anti-CD3,4 bispecific mAbs and highly enriched for CD8⁺ cells; 8, PBLs grown with anti-CD3,4 bispecific mAbs and highly enriched for CD8⁺ cells; 9, cytotoxic clone AB-1.