Table 2.

hFIX production in various cells types

Cell type	hFIX production, ng per 106 cells per day
	AAV-LIXSN vector	LIXSN vector
HeLa	56  ±  15
NIH 3T3	22  ±  6
HepG2	24  ±  15
HFF	520  ±  170	960  ±  210
RDF	450  ±  50	1460  ±  290

Transduced cells were selected for G418 resistance and were grown to 80–90% confluence before changing the medium and collecting a sample for hFIX ELISA 24 h later. Human foreskin fibroblasts (HFF) and rat dermal fibroblasts (RDF) are primary fibroblasts, while the other cell types are continuous cell lines. Each value represents the mean ± SD for two experiments except the value for NIH 3T3 cells, which is the mean of duplicate samples in one experiment.