Table 3.
Wild-type AAV is required for maximal liver transduction by an AAV vector after γ-irradiation
| AAV iu/ml
|
AP+ foci/cm2 | Increase, -fold |
|---|---|---|
| AP+ ffu/ml | ||
| 0.06 | 160 ± 80 (n = 3) | 1 |
| 0.11 | 530 ± 200 (n = 3) | 3.3 |
| 50 | 1040 ± 220 (n = 2) | 6.5 |
Mice received vector stocks containing the indicated ratio of AAV infectious units (iu, as determined by an AAV replication center assay) relative to CWRAPSP ffu. Each mouse received 5 × 105 ffu of CWRAPSP supplemented with various amounts of wild-type AAV2 by tail vein injection after 17 Gy of localized irradiation. Livers were fixed and stained for AP on day 6 after treatment. Results are mean numbers ± SD.