Figure 2.

Mobility-shift analysis of transcription factor binding using specific antibodies (Ab). Wild-type (WT) and TNFR-I knockout mice (TNFR-I KO) were partially hepatectomized and killed 4 h after the operation for NF-κB, STAT3, and AP-1 analysis. Nuclear extracts were incubated for 30 min with ³²P-labeled probes followed by addition of 1 μl of the antibody (1 μg/μl) to 10 μg of extract. After incubation for 30 min, nuclear extracts (10 μg per lane) were analyzed by EMSA. The antibody used in each assay is indicated at the top of the figure. For each transcription factor, the first lane shows EMSA of the nuclear extract without antibody preincubation.