Figure 5.

IL-6 mRNA expression after PH. (A) Wild-type (WT) and TNFR-I knockout (TNFR-I KO) mice were partially hepatectomized and killed 0.5–5 h after the operation, as indicated at the top of A. M, molecular weight marker lane; Pos, positive control for IL-6 mRNA (CLONTECH); Neg, mRNA not added for reverse transcription step. (B) Quantitation of IL-6 mRNA by competitive PCR in livers of wild-type and knockout mice 4 h after PH. Competitor concentrations are indicated at the top of B. The product sizes are 638 bp for IL-6 mRNA and 435 bp for the competitive fragment.