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. 1997 Feb 18;94(4):1447–1452. doi: 10.1073/pnas.94.4.1447

Figure 2.

Figure 2

PCR analysis of LMP1 DNA. (A) LMP1 DNAs in supernatants from lytically infected LCLs coinfected with P3HR-1 and Flag LMP1 recombinant EBV (F-L2 in lane 1) or P3HR-1 and Flag LMP1Δ185–211 recombinant EBV (F-ΔL1 to F-ΔL5 in lanes 2–6) were analyzed by PCR with ΔL5′ (5′-ctctattggttgatctcctttgg-3′) and 3′L315 (5′-attgtggagggcctccatcatttc-3′). Control DNAs pL Flag LMP1 (lane 7) and pL Flag LMP1Δ185–211 (lane 8), and DNA from EBV-negative BJAB cells (lane 9) were also analyzed. Size standards in base pairs are noted to the left. (B). PCR detection of wt LMP1 DNA with 5′L1 (5′-cacgcgttactctgacgtagccg-3′) and WTND (5′-tcctcgagggggccgtcgc-3′). Lanes 1–7 contain 104 IB4 cells (4 genomes per cell) serially 10-fold diluted with 104 EBV-negative BJAB cells. The endpoint lies after lane 5 for a sensitivity of 4 wt LMP1 DNAs per 104 cells. wt LMP1 DNA is in LCL F-L2 (lane 9) but not in LCL F-L1 (lane 8) or LCLs F-L2.1 and F-L2.2 (lanes 10 and 11). Size standards in base pairs are to the left.