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. 1997 Feb 18;94(4):1465–1470. doi: 10.1073/pnas.94.4.1465

Figure 3.

Figure 3

Pax-6 in mouse embryonic nuclear extracts binds to the PBS. (A) Nuclear extracts from E11.5 embryos incubated with the PBS probe (lanes 1 and 3–6) or PBS/DM (lane 2) formed complexes indicated by an arrow. In lanes 3–6, a 100- or 250-fold molar excess of unlabeled PBS (lanes 3 and 4) or PBS/DM (lanes 5 and 6) was added as competitor. (B) Embryonic extracts were incubated with PBS probe and 5 μl of either a 1:10 (lane 2) or 1:100 (lane 3) dilution of anti-Pax-6 rabbit serum or a 1:10 dilution of normal rabbit serum (RS) (lane 4).