Figure 4.

Mutations in PBS-1 and PBS-2 decrease N-CAM promoter activity in a N2A cell line stably expressing Pax-6. Cells were transfected with 0.5 μg of CMVβ and 2 μg of either a CAT gene construct driven by the −414/−15 N-CAM, a similar construct (designated −414/−15 M12) with mutations in PBS-1 and PBS-2, or the promoterless CAT gene (pCATbasic). CAT assays were performed in duplicate. The numbers refer to the relative levels of CAT activity produced by each construct.