Table 1.
α7 wild-type-like and α7 mutant-like properties of hybrid AcChoRs expressed in oocytes injected with a mixture of WT α7 and L247T α7 cDNAs
| nAcChoR type | N | 5HT action | Sensitivity | Desensitization | Rectification |
|---|---|---|---|---|---|
| a | 4/2 | M | M (15–38%) | M (27–126%) | M (56–100%) |
| b | 3/1 | WT | WT (0.4–3%) | WT (1.3–2%) | WT (0.8–5%) |
| c | 7/3 | M | WT (0–6%) | M (7–13%) | M (65–100%) |
| d | 8/4 | WT | WT (0–3%) | M (7–27%) | M (33–100%) |
| e | 3/1 | WT | WT (1.8–2%) | WT (1.2–1.3%) | M (23–63%) |
| f | 1/1 | WT | M (30%) | M (20%) | M (25%) |
| g | 3/2 | WT | WT (1.5–4%) | M (7–12%) | WT (5–6%) |
| h | 2/2 | M | WT (3–4%) | M (13–18%) | WT (5–7%) |
N, number of oocytes/number of donors. M, mutant-like properties. WT, wild-type-like properties. Numbers in parentheses, range of variation. For 5HT action M and WT indicate 5HT acting as agonist or antagonist, respectively. For sensitivity M and WT mean [(I0.2/I100) × 100] ≥ 14% or ≤ 6%, respectively; in desensitization, they signify t1/2 n-fold increase ≥4.5% or ≤2.5% (compared with α7 wild type), respectively; and in rectification, nγ ≥ 23 or ≤ 9, respectively. Upper and lower limits for M and WT properties were established by considering mean values (±3σ) of the parameters for the control homomeric nAcChoRs. cDNAs ratio, 1:1.