Figure 1.
Immunoassays to forms of Aβ and APP described in the text. Immunoassays were prepared to measure APP-α/FL, APP-β (secreted APP ending at the methionine preceding the start of the Aβ region), and total Aβ. Antibodies are mouse mAbs except 192, which is an affinity-purified rabbit polyclonal antibody. Antibodies 192 and 21F12 are specific to fragments of APP and Aβ, respectively, with carboxyl termini as indicated. Antibody 3D6 is specific for Aβ1–5 and does not cross react with APP-α/FL. Methodologies used for the immunoassays are described in text.