Figure 3.

Levels of APP and Aβ in brain regions of heterozygous and homozygous PDAPP transgenic mice. Two-month-old PDAPP mice were sacrificed, and the amount of APP-FL was measured by Western blot analysis using an APP carboxyl-terminal antibody (anti-6; ref. 6) in hippocampus, cortex, cerebellum, and thalamus of heterozygous and homozygous mice. Essentially similar results were obtained from Western blot analyses using other APP antibodies (human-specific 8E5 and 2H3; data not shown). The amount of Aβ in these same regions from eight heterozygous and three homozygous 2-month-old animals was determined by ELISA, and the average levels are listed.