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Effects of PLC inhibitors on GnRH-induced PEt production in GT1 neurons. (A) Dose-dependent inhibition of PEt production by U73122. (B) Inhibitory actions of U73122 (10 μg) and neomycin (1.5 mM) on GnRH (100 nM)-induced PEt production. Cells were stimulated with GnRH for 90 min. U73122 and neomycin were added 5 min before GnRH.
