Lifetime of QB•− or QA•−/S2,3 charge recombination in spinach thylakoids and leaf disks. Samples were exposed to one saturating light flash at 20°C followed by dark interval periods from 0 to 120 s. At the end of the dark period the samples were rapidly cooled (less than 5 s to reach 5°C) and frozen to −40°C after which the residual TL signal was measured. A total of 40 μg Chl/sample or three pea leaf disks, 0.6 cm diameter, were used per measurement. ▴, Leaf disks; ▪, thylakoids; •, thylakoids with addition of DCMU. ñ