Figure 3.

Labeling of pea mitochondrial proteins with [2-¹⁴C]malonic acid. Pea mitochondria were labeled with [2-¹⁴C]malonic acid, and proteins were separated by SDS/PAGE, blotted onto nitrocellulose membrane, and analyzed by PhosphorImager. (a) Time course of labeling of mitochondrial proteins and effect of ATP and MgCl₂. In lanes 2–5, mitochondria were labeled for 10, 20, 40, and 60 min, respectively. In lanes 6 and 7, mitochondria were labeled for 1 h, but ATP or MgCl₂, respectively, were omitted from assay medium. Lane 1 shows a standard of ¹⁴C-labeled 16:0-mtACP. (b) Effects of DTT treatment on deacylation of labeled proteins and cerulenin on labeling of proteins. Pea mitochondria were labeled for 1 h. Lane 1, control that was not treated with DTT; lane 2, mitochondrial proteins were treated with DTT; lanes 3 and 4, 200 μM and 20 μM cerulenin, respectively, were added to the incubation medium, and samples were not treated with DTT.