Figure 5.

Labeling of Neurospora mitochondria with [2-¹⁴C]malonic acid. Neurospora mitochondria were labeled with [2-¹⁴C]malonic acid for 1 h. Proteins without DTT treatment or proteins treated with DTT were separated by SDS/PAGE, blotted onto nitrocellulose membrane, and analyzed by PhosphorImager. (a) Autoradiogram of mitochondrial proteins. Lanes 1 and 2, proteins without DTT treatment and the proteins treated with DTT, respectively. (b) Western blot analysis of mitochondrial proteins probed with antibodies to pea H protein. Lanes 1 and 2, proteins without DTT treatment and the proteins treated with DTT, respectively. The same blot used for autoradiography in a was used for Western blot analysis.