Figure 1.

Identification and characterization of hVIP/MOV34. (a) Amino acid sequence of hVIP/MOV34. Secondary structure prediction is shown above the sequence. Leucine (L) or Isoleucine (I) repeats are underlined in the sequence. Northern blot of poly(A)⁺ RNA from a variety of human organs (b) and RNA from CEM and RD cells and PBMCs (c) were probed with a ³²P-labeled hVIP/MOV34 fragment. (d) hVIP/MOV34 expression was detected by an immunoprecipitation assay. hVIP/MOV34 cDNA was fused in-frame with an anti-Xpress epitope tagged with a six-His mammalian expression vector pcDNA3.His (Invitrogen). HeLa cells were transfected with hVIP/MOV34 expression vectors and then labeled with a ³⁵S protein labeling mixture (NEN), and hVIP/MOV34 was immunoprecipitated with anti-Xpress antibody and analyzed by SDS/PAGE on 15% gels.