Figure 5.

Antisense hVIP/MOV34 inhibits cellular proliferation. (a) Human embryonal rhabdomyosarcoma (RD) cells were transfected with Vpr, hVIP/MOV34 sense, and antisense expression vectors, and the cells were maintained in DMEM containing puromycin (2 μg/ml). The cells were photographed 5–7 days later with a Nikon phase-contrast microscope. (a) Control vector pBabepuro. (b) Vpr. (c) hVIP sense. (d) hVIP/MOV34 antisense. (b) The transfected RD cells were stained with propidium iodide and used for flow cytometric analysis as described (14). RD cells expressing Vpr and hVIP/MOV34 antisense arrest at G₂/M phase of the cell cycle with 4n DNA content.