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. 2003 Oct 1;112(7):989–998. doi: 10.1172/JCI17970

Figure 3.

Figure 3

Stat3-C suppresses Fas-induced apoptotic cell death in liver. (a) Fluoromicroscopic photographs of liver sections with TUNEL staining from PS, LacZ, Stat3-C, and LS3-KO mice treated with Fas agonist (formalin-fixed, paraffin-embedded sections; original magnification, ×100). (b) The DNA ladder pattern was analyzed 6 hours after Fas agonist. Each photo is representative of at least three independent experiments. (c) Apoptotic cell death was quantitatively measured by ELISA. Pretreatment with the general caspase inhibitor Z-VAD-fmk (1 μg/g body wt) or the antioxidant NAC (200 mg/kg body wt) was performed 1 hour prior to the experiments. *P < 0.01 vs. LacZ/Fas agonist, **P < 0.05 vs. LacZ/Fas agonist and Stat3-C/Fas agonist, ***P < 0.05 vs. LacZ/Fas agonist + caspase inhibitor and LacZ/Fas agonist + NAC. (d) Dose-dependent effects of caspase inhibitor (Z-VAD-fmk) or antioxidant (NAC) upon hepatic apoptosis after Fas agonist. *P < 0.01 vs. untreated control, **P < 0.05 vs. Fas agonist, ***P < 0.05 vs. Fas agonist + caspase inhibitor (1 and 2 mg/kg, respectively). All data are expressed as mean ± SEM (n = 5). Casp inh, caspase inhibitor.