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. 2003 Oct 1;112(7):989–998. doi: 10.1172/JCI17970

Figure 4.

Figure 4

Stat3-C induces apoptosis-related proteins and redox-related protein. Immunoblot, Northern blot, and multiprobe ribonuclease protection assay were performed on liver tissues from PS, LacZ, and Stat3-C mice. Sequential changes of the protein amounts after Fas agonist treatment were analyzed in LacZ and Stat3-C mice. Each blot is representative of three independent experiments. (a) Expression of Fas, Bcl-related proteins, and Survivin. (b) Expression of caspase-related proteins. (c) Enzyme activity assays of caspase-3 and caspase-8. *P < 0.05 vs. LacZ, **P < 0.01 vs. LacZ/Fas agonist. (d) Protein expression of redox-related proteins Ref-1, thioredoxin, and MnSOD. (e) Multiprobe ribonuclease protection assay for apoptosis-related genes and redox-related genes 48 hours after adenoviral gene transfer. m, mouse; h, human. (f) Northern blot analysis for Ref-1 mRNA after adenoviral gene transfer of Stat3-C. Results are from a single representative experiment that was reproduced once (d–f). (g) Transcriptional activity of Ref-1 promoter by Stat3-C. Luciferase assay for Ref-1 promoter activity stimulated by AxCAS3-C or AdLacZ. Control was pGL3 without Ref-1 promoter. *P ≤ 0.01 vs. uninfected (0 MOI, 0) and LacZ (25 MOI), **P ≤ 0.01 vs. uninfected (0 MOI) and LacZ (125 MOI). All data are expressed as mean ± SEM (n = 5).