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EPO-mediated activation of critical intracellular kinase cascades including cell survival pathways. Shown are representative Western blots for ERK (a), Akt (b), Jak1 (c), and STAT3 (d) over serial time points. Activated (p-) signaling intermediates were assessed using anti-phospho Ab’s, and activated forms were normalized to total protein content of the corresponding molecule (lower panel of all blots). Data are representative of n = 3–4 experiments.
