Figure 6.

TUNEL staining and quantification of apoptosis in vivo in postischemic rabbit hearts. (a) Graphic representation of TUNEL-positive nuclei in MI + saline (control) rabbit hearts (black bar, n = 4) and MI + EPO rabbits (white bar, n = 4). Data shown are the mean ± SEM of the number of TUNEL-positive cells (nuclei) per high-power field analyzed by microscopy. *P < 0.05 versus MI + saline (control) rabbits. (b) Representative TUNEL-stained LV section from an MI + saline (control) rabbit showing several positive-stained apoptotic nuclei. (c) Representative TUNEL-stained LV section from a MI + EPO rabbit with very few apoptotic nuclei.