Figure 7.

In vivo activation of signaling kinases in the intact adult rabbit heart after EPO treatment. Shown in graphic representation is the activation of (a) Akt and (b) ERK assessed in LV lysates prepared 12 hours after the administration of EPO (1,000 U/kg) or saline (Control). Activated Akt and ERK were assessed using anti-phospho Ab’s, and activated forms were normalized to total protein content of the corresponding molecule as in Figure 1. *P < 0.05 versus control values (n = 4 each).